ANTIOXIDANT ACTIVITY TEST OF ETHANOL EXTRACT OF BANGLE RHIZOME (ZINGIBER CASSUMUNAR ROXB) USING ABTS METHOD [2,2-AZINOBIS (3-ETHYLBENZOTHIAZOLINE)-6-SULFONIC ACID]
Abstract
Antioxidants are compounds that can inhibit or prevent damage caused by free radical oxidation. Bangle rhizome (Zingiber cassumunar Roxb.) contains polyphenolic compounds which have antioxidant activity. This study aims to determine the antioxidant activity of bangle rhizome extract in reducing ABTS radicals [2,2'-azino-bis-(3-ethylbenzothiazoline)-6-sulphonic acid]. Bangle rhizome was macerated with 96% ethanol. The extract identified flavonoid and tested for antioxidant activity using the ABTS method with comparison quercetin. The results of the antioxidant activity test of bangle rhizome had an IC50 value of 25.320 ppm which was classified as very strong antioxidant, while the positive control of quercetin obtained an IC50 value of 3.394 ppm which was included in the very strong category. Bangle rhizome extract has very strong antioxidant activity in reducing free radicals.
References
Citradewi, A. Sumarya, I.M,. Juliansih, N. K. . (2019). DAYA HAMBAT EKSTRAK RIMPANG BANGLE (Zingiber purpureum Roxb.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus. 01.
Faisal, H. (2019). Uji Aktivitas Antioksidan Ekstrak Etanol Buah Okra ( Abelmoschus esculentus L . Moench ) Dengan Metode DPPH ( 1 , 1- difenil-2-pikrilhidrazil ) dan Metode ABTS. Regional Development Industry & Health Science, Technology and Art of Life, 2 (1), 1–5.
Fitriana, W. D., Fatmawati, S., & Ersam, T. (2015). Uji Aktivitas Antioksidan terhadap DPPH dan ABTS dari Fraksi-fraksi. SNIP Bandung, 2015(Snips), 658.
Kusuma, P. (2012). Penetapan Kadar Flavonoid Total dan Daya Antioksidan dari Ekstrak Etanol Buah Pare (Momordica charantia L). Skripsi Fakultas Ilmu Kesehatan, Universitas Islam Negeri Alaudin, Makassar, 1–26. http://repositori.uin-alauddin.ac.id/1957/
Molyneux P. (2004). The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating anti-oxidant activity. Songklanakarin Journal of Science and Technology, 26(May), 211–219.
P.Saranraj. (2011). PHARMACOLOGICAL SCREENING OF Datura metel AND Acalypha indica FOR ITS ANTIFUNGAL ACTIVITY AGAINST PATHOGENIC FUNGI. 13(1), 43–50. http://dx.doi.org/10.1038/ni.1913%0Ahttp://dx.doi.org/10.1016/j.dci.2013.08.014%0Ahttp://dx.doi.org/10.1186/s13071-016-1819-4%0Ahttp://dx.doi.org/10.1016/j.actatropica.2017.02.006%0Ahttp://dx.doi.org/10.1038/s41598-017-09955-y%0Ahttp://dx.doi.org/10.1016/
Pantria Saputri, A., Augustina, I., & Fatmaria. (2020). UJI AKTIVITAS ANTIOKSIDAN EKSTRAK AIR KULIT PISANG KEPOK (Musa acuminate x Musa balbisiana (ABB cv)) DENGAN METODE ABTS (2,2 azinobis (3-etilbenzotiazolin)-6-asam sulfonat) PADA BERBAGAI TINGKAT KEMATANGAN. Jurnal Kedokteran Universitas Palangka Raya, 8(1), 973–980. https://doi.org/10.37304/jkupr.v8i1.1502
Rahmadani, S., Siti Sa’diah, & Sri Wardatun. (2008). Optimasi ekstraksi jahe merah (. Teknologi Pangan, 1(2), 1–8.
Rissanti, I., Fachriyah, E., & Kusrini, D. (2014). Isolasi dan Identifikasi Senyawa Aktif dari Ekstrak Aseton Rimpang Bangle (Zingiber cassumunar Roxb.) sebagai Antioksidan. Jurnal Kimia Sains Dan Aplikasi, 17(3), 75–79. https://doi.org/10.14710/jksa.17.3.75-79
Sami, F. J., & Rahimah, S. (2016). UJI AKTIVITAS ANTIOKSIDAN EKSTRAK METANOL BUNGA BROKOLI (Brassica oleracea L. var. Italica) DENGAN METODE DPPH (2,2 diphenyl-1-picrylhydrazyl) dan METODE ABTS (2,2 azinobis (3-etilbenzotiazolin)-6-asam sulfonat). Jurnal Fitofarmaka Indonesia, 2(2), 107–110. https://doi.org/10.33096/jffi.v2i2.179
Serhan, M., Sprowls, M., Jackemeyer, D., Long, M., Perez, I. D., Maret, W., Tao, N., & Forzani, E. (2019). Total iron measurement in human serum with a smartphone. AIChE Annual Meeting, Conference Proceedings, 2019-Novem. https://doi.org/10.1039/x0xx00000x
Shalaby, E. A., Shanab, S. M. M., & Singh, V. (2010). Salt stress enhancement of antioxidant and antiviral efficiency of Spirulina platensis. Journal of Medicinal Plants Research, 4(24), 2622–2632. https://doi.org/10.5897/jmpr09.300
Vifta, R. L., Rahayu, R. T., & Luhurningtyas, F. P. (2019). Uji Aktivitas Antioksidan Kombinasi Ekstrak Buah Parijoto (Medinilla Speciosa) dan Rimpang Jahe Merah (Zingiber Oficinalle) dengan Metode ABTS (2,2-Azinobis (3-Etilbenzotiazolin)-6-Asam Sulfonat). Indonesian Journal of Chemical Science, 8(3), 197–201.
Faisal, H. (2019). Uji Aktivitas Antioksidan Ekstrak Etanol Buah Okra ( Abelmoschus esculentus L . Moench ) Dengan Metode DPPH ( 1 , 1- difenil-2-pikrilhidrazil ) dan Metode ABTS. Regional Development Industry & Health Science, Technology and Art of Life, 2 (1), 1–5.
Fitriana, W. D., Fatmawati, S., & Ersam, T. (2015). Uji Aktivitas Antioksidan terhadap DPPH dan ABTS dari Fraksi-fraksi. SNIP Bandung, 2015(Snips), 658.
Kusuma, P. (2012). Penetapan Kadar Flavonoid Total dan Daya Antioksidan dari Ekstrak Etanol Buah Pare (Momordica charantia L). Skripsi Fakultas Ilmu Kesehatan, Universitas Islam Negeri Alaudin, Makassar, 1–26. http://repositori.uin-alauddin.ac.id/1957/
Molyneux P. (2004). The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating anti-oxidant activity. Songklanakarin Journal of Science and Technology, 26(May), 211–219.
P.Saranraj. (2011). PHARMACOLOGICAL SCREENING OF Datura metel AND Acalypha indica FOR ITS ANTIFUNGAL ACTIVITY AGAINST PATHOGENIC FUNGI. 13(1), 43–50. http://dx.doi.org/10.1038/ni.1913%0Ahttp://dx.doi.org/10.1016/j.dci.2013.08.014%0Ahttp://dx.doi.org/10.1186/s13071-016-1819-4%0Ahttp://dx.doi.org/10.1016/j.actatropica.2017.02.006%0Ahttp://dx.doi.org/10.1038/s41598-017-09955-y%0Ahttp://dx.doi.org/10.1016/
Pantria Saputri, A., Augustina, I., & Fatmaria. (2020). UJI AKTIVITAS ANTIOKSIDAN EKSTRAK AIR KULIT PISANG KEPOK (Musa acuminate x Musa balbisiana (ABB cv)) DENGAN METODE ABTS (2,2 azinobis (3-etilbenzotiazolin)-6-asam sulfonat) PADA BERBAGAI TINGKAT KEMATANGAN. Jurnal Kedokteran Universitas Palangka Raya, 8(1), 973–980. https://doi.org/10.37304/jkupr.v8i1.1502
Rahmadani, S., Siti Sa’diah, & Sri Wardatun. (2008). Optimasi ekstraksi jahe merah (. Teknologi Pangan, 1(2), 1–8.
Rissanti, I., Fachriyah, E., & Kusrini, D. (2014). Isolasi dan Identifikasi Senyawa Aktif dari Ekstrak Aseton Rimpang Bangle (Zingiber cassumunar Roxb.) sebagai Antioksidan. Jurnal Kimia Sains Dan Aplikasi, 17(3), 75–79. https://doi.org/10.14710/jksa.17.3.75-79
Sami, F. J., & Rahimah, S. (2016). UJI AKTIVITAS ANTIOKSIDAN EKSTRAK METANOL BUNGA BROKOLI (Brassica oleracea L. var. Italica) DENGAN METODE DPPH (2,2 diphenyl-1-picrylhydrazyl) dan METODE ABTS (2,2 azinobis (3-etilbenzotiazolin)-6-asam sulfonat). Jurnal Fitofarmaka Indonesia, 2(2), 107–110. https://doi.org/10.33096/jffi.v2i2.179
Serhan, M., Sprowls, M., Jackemeyer, D., Long, M., Perez, I. D., Maret, W., Tao, N., & Forzani, E. (2019). Total iron measurement in human serum with a smartphone. AIChE Annual Meeting, Conference Proceedings, 2019-Novem. https://doi.org/10.1039/x0xx00000x
Shalaby, E. A., Shanab, S. M. M., & Singh, V. (2010). Salt stress enhancement of antioxidant and antiviral efficiency of Spirulina platensis. Journal of Medicinal Plants Research, 4(24), 2622–2632. https://doi.org/10.5897/jmpr09.300
Vifta, R. L., Rahayu, R. T., & Luhurningtyas, F. P. (2019). Uji Aktivitas Antioksidan Kombinasi Ekstrak Buah Parijoto (Medinilla Speciosa) dan Rimpang Jahe Merah (Zingiber Oficinalle) dengan Metode ABTS (2,2-Azinobis (3-Etilbenzotiazolin)-6-Asam Sulfonat). Indonesian Journal of Chemical Science, 8(3), 197–201.
Published
2022-04-23
How to Cite
Djide, M., Mubarak, F., & Natasya, R. (2022). ANTIOXIDANT ACTIVITY TEST OF ETHANOL EXTRACT OF BANGLE RHIZOME (ZINGIBER CASSUMUNAR ROXB) USING ABTS METHOD [2,2-AZINOBIS (3-ETHYLBENZOTHIAZOLINE)-6-SULFONIC ACID]. Jurnal Inovasi Pendidikan Dan Sains, 3(1), 28-32. https://doi.org/10.51673/jips.v3i1.941
Issue
Section
Artikel
